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Home > Learn > Pregnancy > >Natalist Pregnancy Test Instructions

Natalist Pregnancy Test Instructions

Apr 29, 20 7 min

About the Natalist Pregnancy Test

How does this test work? It detects a hormone called human Chorionic Gonadotropin (hCG), the first detectable sign of pregnancy. An embryo makes hCG, which can be detected in urine after it implants into the uterine wall. If the test detects hCG, it will show a positive result.

You can use the Natalist Pregnancy Test starting 5 days before you expect your period to start. For the most accurate results (over 99%), test as close as you can to your expected period.

Before you test

The Natalist Pregnancy Test is over 99% accurate when used correctly—so be sure to read all the instructions before you start.

It’s best to test first thing in the morning when your urine is most concentrated. If you’re pregnant, there will be more hCG for the test to detect. This is especially important if you’re taking the test before your expected period. If you take it later in the day, make sure you don’t drink a lot of liquid in the hours before testing.


  • Use before the expiration date on the wrapper

  • Don’t open the wrapper until you’re ready to start testing, and don’t use if the wrapper is damaged or torn

  • Store between 36-86°F (2-30°C) 

  • Don’t freeze this test

  • Keep out of children’s reach

  • Don’t use this test internally. For in vitro diagnostic use only. (In other words, if you put this test in your vagina, you’re doing it wrong.)

  • Not for contraceptive use

  • Use once


Natalist pregnancy test instructions

 Taking the test

  1. Find a well-lit bathroom with a clean, flat surface.

  2. Unwrap 1 test and take the cap off to reveal the absorbent tip. Save the cap nearby—you’ll use it again soon.

  3. Sit on the toilet and get the test in place. Hold the test handle and point the absorbent tip downward.

  4. Pee directly on the absorbent tip (everything below the arrow) until it’s completely wet (about 5 seconds). Be careful not to pee above the arrow, on the test window, or on your fingers! 

  5. Keep the absorbent tip pointing downwards as you put the cap back on. 

  6. Lay the test on a flat surface with the result window facing up. Wait 5 minutes to look at your results—and make sure to read the results within 15 minutes.

Get the answer you need. As early as possible. Shop pregnancy tests!

Reading your results

The test window can show 2 lines:

  1. The control line (C) to make sure the test worked.

  2. The test line (T) that shows a positive result.
Positive ovulation test lines instructions

If 2 lines show up, even if the test line (T) is very faint, that’s a positive—or pregnant result.

Negative ovulation test lines instructions

If only the control line (C) shows up, the test is negative. Either you’re not pregnant or it’s too early to test.


When will my results show up and how long will they last?

Check your results after 5 minutes—and make sure to read within 15 minutes. After 15 minutes, the results may not be accurate.

One line is darker than the other. Am I pregnant?

If you see 2 lines, even if one is darker than the other, that’s a positive, pregnant result. If your test is positive, see your doctor to talk about what’s next.  

My test line is really faint. What’s going on?

A few possibilities here. If you see the test line (T) at all, that’s a positive, pregnant result. If you’re straining to find a faint line, it may be too early to test or your urine is too watered down. Test again in a few days. 

My test is negative. What does that mean?

If you see a control line (C) but don’t see a test line (T), the test couldn’t detect hCG in your urine. You either don’t have enough hCG in your urine yet, or you’re not pregnant. If you think    you could be pregnant, test again in a couple of days. 

I’m pretty sure I’m pregnant, but the test says otherwise. What should I do?

A negative result means the test did not detect hCG in your urine. A false-negative result (when the pregnancy test is negative, but you’re actually pregnant) can happen for 2 reasons. 

  1. Either your urine was too diluted (don’t drink a lot of liquid for a few hours before testing) or 

  2. It’s too early to detect a pregnancy. If you tested before your expected period, this could be the case (see What day should I test?). If you feel you’re pregnant, test again in a few days. hCG levels soar in early pregnancy—doubling every 48 hours. If you miss your period and still get a negative result, see your doctor.

It’s really rare, but if after 5 minutes you see no lines or only a test line (T), the test either wasn’t saturated with enough urine—or it’s defective or expired (check the wrapper). Throw it out and try again with a new one. Send us a photo of the invalid test (include the wrapper with its printed lot number and expiration date) to, and we’ll help you out.

What can affect my results?

  • Fertility drugs containing hCG (such as Pregnyl* and Profasi**). Drugs containing hCG usually clear your body 2 weeks post-injection.
  • A recent miscarriage or pregnancy, including a chemical pregnancy (a pregnancy loss  very soon after implantation). This may give you a false positive result when you’re no longer pregnant.
  • Rare medical conditions that result in the production of hCG unrelated to pregnancy. These include gestational trophoblastic disease, ovarian tumors, and hCG secreted by the pituitary gland during menopause. If you think this could be you, talk to your doctor.
  • Following instructions incorrectly. 
  • Waiting too long to read your results. Results read after 15 minutes aren’t accurate.

A control line didn't appear. What does that mean?

That's an invalid result. This could mean the absorbent tip wasn’t saturated with enough urine, or the test is expired or damaged. Grab another test and try again. If you still get an invalid result, contact us at 

What time of day should I test?

If you’re testing before your expected period, it’s best to test first thing in the morning (when your urine has a higher concentration of hCG), but you can test any time of the day. Keep in mind that drinking a bunch of liquid in the few hours before testing can dilute your urine and cause a false negative result (meaning you could be pregnant, but the test will show you’re not).

What day should I test?

You can use the Natalist Pregnancy Test starting 5 days before you expect your period to start. For the most accurate results, test on the day of your expected period. If you get a negative result and tested more than 3 days before your expected period, you could still be pregnant (see chart). Take the test again in a few days. Since it would be really early in your pregnancy, you may not have enough hCG for the test to detect yet. In just 2 days, your hCG levels will usually double. The more hCG your body makes, the more likely you are to get a positive result.

Pregnancy test percentage chart

When should I test? 

Fifteen percent of women have irregular periods, which can make it harder to predict when to test. If this is you, try counting at least 14 days from when you had sex. If your test says you’re not pregnant and you think you might be, test again in a few days.

How accurate is the test?

The Natalist Pregnancy Test is more than 99% accurate in clinical studies when used on the day of your expected period. 

How sensitive is the test?

The test can detect the pregnancy hormone hCG with over 99% accuracy at 10 mIU/mL. 

My test is expired. Should I still use it?


Can this test tell me how much hCG is in my urine?

It can’t. This test is for the qualitative detection of hCG in urine—meaning it tells you if hCG is detected in your urine, but not how much. This test also can’t tell you how much your hCG levels have increased over time.

Have more questions?

We’d love to help. Text or email us.



Download a PDF version of these instructions here.

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